In vivo track the development of melanoma with the intrinsic third harmonic generation and two-photon fluorescence contrasts of melanin

Pei-Chun Wu; Yu-Shing Chen; Tsung-Yuan Hsieh; Han-Wen Liu; Wen-Li Lin; Tzu-Ming Liu

doi:10.1117/12.906682

2 February 2012 In vivo track the development of melanoma with the intrinsic third harmonic generation and two-photon fluorescence contrasts of melanin

Pei-Chun Wu, Yu-Shing Chen, Tsung-Yuan Hsieh, Han-Wen Liu, Wen-Li Lin, Tzu-Ming Liu

Author Affiliations +

Proceedings Volume 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV; 82330O (2012) https://doi.org/10.1117/12.906682
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The understanding of the interaction between tumors and surrounding microenvironment in vivo is an important first step and basis for pathway-targeting cancer therapy. To in vivo observe the dynamic development of tumor cells and validate the efficacy of therapy in microscopic scales, people commonly performed multi-photon fluorescence microscopy through an invasive window chamber setup. However, under such system, the cancer cells can't be identified and long-term tracked without a fluorescence labeling. Exploiting the intrinsic third harmonic generation (THG) and two-photon fluorescence (2PF) contrasts of melanin, we demonstrated in vivo identification of melanoma and tracked its development without labeling. It was achieved with a least invasive femtosecond Cr:forsterite laser and a laser scanning nonlinear microscopy system with 3D sub-micron spatial resolution. Combined with molecular probes or reporters, we anticipate thus developed platform a powerful tool to reveal molecular insights of tumor microenvironments, enhance our understanding of tumor biology, and trigger new therapeutic approaches.

Citation Download Citation

Pei-Chun Wu, Yu-Shing Chen, Tsung-Yuan Hsieh, Han-Wen Liu, Wen-Li Lin, and Tzu-Ming Liu "In vivo track the development of melanoma with the intrinsic third harmonic generation and two-photon fluorescence contrasts of melanin", Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82330O (2 February 2012); https://doi.org/10.1117/12.906682

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