Detection of amino acid neurotransmitters by surface enhanced Raman scattering and hollow core photonic crystal fiber

Vidhu S. Tiwari; Altaf Khetani; Ali Momenpour T. Monfared; Brett Smith; Hanan Anis; Vance L. Trudeau

doi:10.1117/12.907754

2 February 2012 Detection of amino acid neurotransmitters by surface enhanced Raman scattering and hollow core photonic crystal fiber

Vidhu S. Tiwari, Altaf Khetani, Ali Momenpour T. Monfared, Brett Smith, Hanan Anis, Vance L. Trudeau

Author Affiliations +

Proceedings Volume 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV; 82330Q (2012) https://doi.org/10.1117/12.907754
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The present work explores the feasibility of using surface enhanced Raman scattering (SERS) for detecting the neurotransmitters such as glutamate (GLU) and gamma-amino butyric acid (GABA). These amino acid neurotransmitters that respectively mediate fast excitatory and inhibitory neurotransmission in the brain, are important for neuroendocrine control, and upsets in their synthesis are also linked to epilepsy. Our SERS-based detection scheme enabled the detection of low amounts of GLU (10^-7 M) and GABA (10^-4 M). It may complement existing techniques for characterizing such kinds of neurotransmitters that include high-performance liquid chromatography (HPLC) or mass spectrography (MS). This is mainly because SERS has other advantages such as ease of sample preparation, molecular specificity and sensitivity, thus making it potentially applicable to characterization of experimental brain extracts or clinical diagnostic samples of cerebrospinal fluid and saliva. Using hollow core photonic crystal fiber (HC-PCF) further enhanced the Raman signal relative to that in a standard cuvette providing sensitive detection of GLU and GABA in micro-litre volume of aqueous solutions.

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Vidhu S. Tiwari, Altaf Khetani, Ali Momenpour T. Monfared, Brett Smith, Hanan Anis, and Vance L. Trudeau "Detection of amino acid neurotransmitters by surface enhanced Raman scattering and hollow core photonic crystal fiber", Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82330Q (2 February 2012); https://doi.org/10.1117/12.907754

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