Calibrating the imaging and therapy performance of magneto-fluorescent gold nanoshells for breast cancer

Adam Dowell; Wenxue Chen; Nrusingh Biswal; Ciceron Ayala-Orozco; Mario Giuliano; Rachel Schiff; Naomi J. Halas; Amit Joshi

doi:10.1117/12.909066

24 February 2012 Calibrating the imaging and therapy performance of magneto-fluorescent gold nanoshells for breast cancer

Adam Dowell, Wenxue Chen, Nrusingh Biswal, Ciceron Ayala-Orozco, Mario Giuliano, Rachel Schiff, Naomi J. Halas, Amit Joshi

Author Affiliations +

Proceedings Volume 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV; 82330W (2012) https://doi.org/10.1117/12.909066
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Gold nanoshells with NIR plasmon resonance can be modified to simultaneously enhance conjugated NIR fluorescence dyes and T2 contrast of embedded iron-oxide nanoparticles, and molecularly targeted to breast and other cancers. We calibrated the theranostic performance of magneto-fluorescent nanoshells, and contrasted the performance of molecularly targeted and untargeted nanoshells for breast cancer therapy, employing MCF-7L and their HER2 overexpressing derivative MCF-7/HER2-18 breast cancer cells as in vitro model systems. Silica core gold nanoshells with plasmon resonance on ~810 nm were doped with NIR dye ICG and ~10 nm iron-oxide nanoparticles in a ~20 nm epilayer of silica. A subset of nanoshells was conjugated to antibodies targeting HER2. Cell viability with varying laser power levels in presence and absence of bare and HER2-targeted nanoshells was assessed by calcein and propidium iodide staining. For MCF-7L cells, increasing power resulted in increased cell death (F=5.63, p=0.0018), and bare nanoshells caused more cell death than HER2-targeted nanoshells or laser treatment alone (F=30.13, p<0.001). For MCF-7/HER2-18 cells, death was greater with HER2-targeted nanoshells and was independent of laser power. This study demonstrates the capability of magneto-fluorescent nanocomplexes for imaging and therapy of breast cancer cells, and the advantages of targeting receptors unique to cancer cells.

Citation Download Citation

Adam Dowell, Wenxue Chen, Nrusingh Biswal, Ciceron Ayala-Orozco, Mario Giuliano, Rachel Schiff, Naomi J. Halas, and Amit Joshi "Calibrating the imaging and therapy performance of magneto-fluorescent gold nanoshells for breast cancer", Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82330W (24 February 2012); https://doi.org/10.1117/12.909066

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available