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14 February 2012 Fast self-assembly kinetics of alkanethiols on gold nanoparticles: simulation and characterization by localized surface plasmon resonance spectroscopy
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Abstract
This study demonstrates improved kinetics for the formation of self-assembled monolayers (SAMs) of alkanethiols on gold nanoparticle substrates. A computational model was developed to predict SAM growth kinetics. Based on the predictions from the model, SAMs of 11-mercaptoundecanoic acid (11-MUA) and 1-octanethiol (1-OT) were formed by incubation of gold nanoparticle chips in an ethanolic 10 mM solution within 20 min. The performance of this novel rapid SAM formation protocol was compared with a conventional 24 hour incubation protocol. Binding capacity of the alkanethiol SAM was investigated for a 20 min incubation protocol using biotin-streptavidin. For this purpose, the SAM loaded gold nanoparticle chips were modified with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) to allow attachment of EZ-Link amine PEG3 biotin to the 11-MUA molecules. Binding reactions were monitored in real time using localized surface plasmon resonance (LSPR) spectroscopy. The resulting LSPR absorbance peak shift was comparable to the experimental results for biotin-streptavidin reported in literature. Results of this study suggest that formation of a high quality alkanethiol SAM within 20 min on gold nanoparticles surfaces is possible and could greatly reduce the time and cost compared to conventional 24 h incubation protocols.
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Sasan Asiaei, Ryan C. Denomme, Chelsea Marr, Patricia M.. Nieva, and Mathilakath M. Vijayan "Fast self-assembly kinetics of alkanethiols on gold nanoparticles: simulation and characterization by localized surface plasmon resonance spectroscopy", Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 825107 (14 February 2012); https://doi.org/10.1117/12.907370
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