A new approach for real-time analysis of biomolecular interactions using surface plasmon resonance imaging SPRi

H. Mezlini; C. Fetita; M. Canva; J. Moreau; H. Ghalila; S. Ghalila

doi:10.1117/12.911410

16 April 2012 A new approach for real-time analysis of biomolecular interactions using surface plasmon resonance imaging SPRi

H. Mezlini, C. Fetita, M. Canva, J. Moreau, H. Ghalila, S. Ghalila

Author Affiliations +

Proceedings Volume 8317, Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging; 831725 (2012) https://doi.org/10.1117/12.911410
Event: SPIE Medical Imaging, 2012, San Diego, California, United States

Abstract

The real-time monitoring of different molecular interactions can be used as a lower cost tool for genetic diagnosis. The extraction of the hybridization signal allows the estimation of the association/dissociation constants, the affinity of biomolecular components (target/probe) that interact and then characterize their activities and functions. This extraction of the biological information is based on the analysis of images acquired by a CCD camera during the course of the experiment and a self-calibration of the data obtained. Until now, the processing of these images was post experimental and concerned different stages of analysis: the detection of spots region, spatiotemporal segmentation of areas of interaction and eventually the quantification of these areas using the kinetic response measured. The challenging issue is to continue to improve the automatic extraction of the interaction signal and develop a processing tool applied in real-time as the image acquisition progresses. The advantage of such treatment is to allow the prediction of the evolution of the interaction, especially in the case of genetic diagnosis. It may also detect any malfunction that may arise during the interaction and allow the experimenter to decide whether to continue or interrupt the experience. This paper proposes a new approach for the real-time analysis of the image data provided by the SPR. A self-calibration step allows the correction of microarray design flaws or of temporal artifacts. Once the data are normalized, 3D morphological operators are used to extract the binary mask that will allow detecting all regions of interest for dynamic segmentation. This segmentation is then used in a spatio-temporal classification to extract the effective signal within each detected spot. The resulting real-time analysis approach presents a great interest in genetic diagnosis applications.

Citation Download Citation

H. Mezlini, C. Fetita, M. Canva, J. Moreau, H. Ghalila, and S. Ghalila "A new approach for real-time analysis of biomolecular interactions using surface plasmon resonance imaging SPRi", Proc. SPIE 8317, Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging, 831725 (16 April 2012); https://doi.org/10.1117/12.911410

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
14 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Calibration

Image segmentation

Biological research

Image analysis

Image processing

Data acquisition

Reflectivity

Show All Keywords

Keywords/Phrases

Search In:

Publication Years