Diagnostic potential for gold nanoparticle-based surface-enhanced Raman spectroscopy to provide colorectal cancer screening using blood serum sample

Duo Lin; Shangyuan Feng; Jianji Pan; Yanping Chen; Juqiang Lin; Liqing Sun; Rong Chen

doi:10.1117/12.919003

13 March 2012 Diagnostic potential for gold nanoparticle-based surface-enhanced Raman spectroscopy to provide colorectal cancer screening using blood serum sample

Duo Lin, Shangyuan Feng, Jianji Pan, Yanping Chen, Juqiang Lin, Liqing Sun, Rong Chen

Author Affiliations +

Proceedings Volume 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011); 83290L (2012) https://doi.org/10.1117/12.919003
Event: Photonics and Optoelectronics Meetings 2011, 2011, Wuhan, China

Abstract

Surface-enhanced Raman spectroscopy (SERS) is a vibrational spectroscopic technique that is capable of probing the biomolecular changes associated with diseased transformation. The objective of our study was to explore gold nanoparticle based SERS to obtain blood serum biochemical information for non-invasive colorectal cancer detection. SERS measurements were performed on two groups of blood serum samples: one group from patients (n = 38) with pathologically confirmed colorectal cancer and the other group from healthy volunteers (control subjects, n = 45). Tentative assignments of the Raman bands in the measured SERS spectra suggested interesting cancer specific biomolecular changes, including an increase in the relative amounts of nucleic acid, a decrease in the percentage of saccharide and proteins contents in the blood serum of colorectal cancer patients as compared to that of healthy subjects. Principal component analysis (PCA) of the measured SERS spectra separated the spectral features of the two groups into two distinct clusters with little overlaps. Linear discriminate analysis (LDA) based on the PCA generated features differentiated the nasopharyngeal cancer SERS spectra from normal SERS spectra with high sensitivity (97.4%) and specificity (100%). The results from this exploratory study demonstrated that gold nanoparticle based SERS serum analysis combined with PCA-LDA has tremendous potential for the non-invasive detection of colorectal cancers.

Citation Download Citation

Duo Lin, Shangyuan Feng, Jianji Pan, Yanping Chen, Juqiang Lin, Liqing Sun, and Rong Chen "Diagnostic potential for gold nanoparticle-based surface-enhanced Raman spectroscopy to provide colorectal cancer screening using blood serum sample", Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290L (13 March 2012); https://doi.org/10.1117/12.919003

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