Effectiveness of multiple pulses on flow index of electroporation

Bashir I. Morshed; Maitham Shams; Tofy Mussivand

doi:10.1117/12.917435

30 March 2012 Effectiveness of multiple pulses on flow index of electroporation

Bashir I. Morshed, Maitham Shams, Tofy Mussivand

Proceedings Volume 8344, Nanosensors, Biosensors, and Info-Tech Sensors and Systems 2012; 834417 (2012) https://doi.org/10.1117/12.917435
Event: SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring, 2012, San Diego, California, United States

Abstract

Electroporation is the formation of reversible pores in cell membranes without rupturing the membrane using a high electric field. Electroporation is an important technique for various biomedical applications including drug delivery, gene transfection and therapeutic treatments. A microfluidic device was developed to investigate electroporation using single and multiple pulses. The device contained integrated electrodes inside microchannels. Stained cells were introduced inside the microchannels and excitation pulses were applied. Sequences of images were captured using an integrated-camera on an optical microscope in the bright-field mode. Stained pixel data from the sequences of images were extracted through image processing to detect and quantify electroporation. Flow Index of EP (FIEP) was computed from the normalized (wrt initial) stained pixel data. Multiple pulses increased FIEP when increased energy was delivered, but reduced FIEP when the same amount of energy was delivered. Mean FIEP using 20 V excitation for 1 pulse of 1 ms was 0.256, 10 pulses of 1 ms was 0.329 and 1 pulse of 10 ms was 0.422. These experimental results show that a single pulse is more effective to induce higher FIEP compared to multiple pulses. FIEP enables quantitative and systematic study towards optimization of pulse parameters for electroporation-based applications.

Citation Download Citation

Bashir I. Morshed, Maitham Shams, and Tofy Mussivand "Effectiveness of multiple pulses on flow index of electroporation", Proc. SPIE 8344, Nanosensors, Biosensors, and Info-Tech Sensors and Systems 2012, 834417 (30 March 2012); https://doi.org/10.1117/12.917435

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