Label free detection of quadruplex formation of peroxidase-like DNAzyme by tapered fiber optic biosensor

Z. Bagheri; Z. Chenari; Z. Karami; M. I. Zibaii; B. Ranjbar; H. Latifi

doi:10.1117/12.966396

17 October 2012 Label free detection of quadruplex formation of peroxidase-like DNAzyme by tapered fiber optic biosensor

Z. Bagheri, Z. Chenari, Z. Karami, M. I. Zibaii, B. Ranjbar, H. Latifi

Author Affiliations +

Proceedings Volume 8421, OFS2012 22nd International Conference on Optical Fiber Sensors; 84215L (2012) https://doi.org/10.1117/12.966396
Event: OFS2012 22nd International Conference on Optical Fiber Sensor, 2012, Beijing, China

Abstract

We investigated the possibility the active DNAzyme formation of particular Guanine-rich oligonucleotide with nonadiabatic tapered optical fiber (NATOF) sensor. These kinds of oligonucleotides can form stable four-stranded structure called G-quadruplex in the presence of particular metal ions. Structural change of immobilized oligonucleotide was detected by monitoring of transmission spectrum of the sensor. The formation of active DNAzyme formed was confirmed by verifying enzyme activity. Result shows that the NATOF sensor has capability to detect intramolecular structural changes and using this sequence as biorecognition molecule for fabricating fiber optic biosensor.

Citation Download Citation

Z. Bagheri, Z. Chenari, Z. Karami, M. I. Zibaii, B. Ranjbar, and H. Latifi "Label free detection of quadruplex formation of peroxidase-like DNAzyme by tapered fiber optic biosensor", Proc. SPIE 8421, OFS2012 22nd International Conference on Optical Fiber Sensors, 84215L (17 October 2012); https://doi.org/10.1117/12.966396

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available