Force and Raman spectroscopy of single red blood cell

Saurabh Raj; Michal Wojdyla; Monica Marro Sanchez; Dmitri Petrov

doi:10.1117/12.921355

8 May 2012 Force and Raman spectroscopy of single red blood cell

Saurabh Raj, Michal Wojdyla, Monica Marro Sanchez, Dmitri Petrov

Proceedings Volume 8427, Biophotonics: Photonic Solutions for Better Health Care III; 842712 (2012) https://doi.org/10.1117/12.921355
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

Viscoelastic and spectroscopic properties of single RBC are probed using dual beam optical tweezers and Raman techniques, respectively. Complex response function of cell was measured by means of one and two particles passive microrheology at different stretching states yielding local and overall mechanical properties of exactly the same human erythrocyte. The frequency dependent response function (measured up to 10 kHz) was corrected for the presence of the traps and spectral distribution of complex stiffness over controlled range of cell deformation is calculated and discussed. The presence of non-thermal sources of membrane motions is also explored based on comparison of passive and active microrheology experiments. In order to get insight into structural changes of RBC due to deformation, Raman spectra of single cell were recorded. Evolution of Raman bands with cell deformation was analyzed using sensitive 2D correlation method. The combination of force and Raman spectroscopy is promising and potentially very powerful method to establish essential linkages between structure, mechanical properties and functions of living cells.

Citation Download Citation

Saurabh Raj, Michal Wojdyla, Monica Marro Sanchez, and Dmitri Petrov "Force and Raman spectroscopy of single red blood cell", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842712 (8 May 2012); https://doi.org/10.1117/12.921355

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available