Investigation of cell morphology by the TRUImagE digital holographic microscopy system

Oi Choo Chee; Weijuan Qu; Kim Kheong Chai; Anand Asundi

doi:10.1117/12.921613

8 May 2012 Investigation of cell morphology by the TRUImagE digital holographic microscopy system

Oi Choo Chee, Weijuan Qu, Kim Kheong Chai, Anand Asundi

Proceedings Volume 8427, Biophotonics: Photonic Solutions for Better Health Care III; 84272G (2012) https://doi.org/10.1117/12.921613
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

We report the development of the Three-dimensional Real-time Uninvasive Imaging and Evaluation (TRUImagE) system based on digital holographic microscopy to study the morphological changes in cells undergoing photodynamic therapyinduced cell death. The optical system, based on the Michelson interferometer and configured in transmission mode, and the sample holder incorporating a stage incubator have been developed for monitoring various tumorigenic cell samples without the use of markers. Off-axis digital holograms were recorded with a CCD sensor and numerically reconstructed to provide quantitative phase imaging and 3D morphology of the cells in real time. The system was used to continuously monitor and study, at different time points, the changes in cells after incubation with the photosensitizer followed by activation by the appropriate light dose. Results obtained from the TRUImagE system and biochemical assays will be given.

Citation Download Citation

Oi Choo Chee, Weijuan Qu, Kim Kheong Chai, and Anand Asundi "Investigation of cell morphology by the TRUImagE digital holographic microscopy system", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84272G (8 May 2012); https://doi.org/10.1117/12.921613

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KEYWORDS

Photodynamic therapy

Digital holography

Cell death

Holography

Microscopy

Imaging systems

Plasma

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