Analysis of cell poration by femtosecond laser for particle insertion by optical manipulation

Waleed Muhammad; Jung-Dae Kim; Yong-Gu Lee

doi:10.1117/12.929332

10 October 2012 Analysis of cell poration by femtosecond laser for particle insertion by optical manipulation

Waleed Muhammad, Jung-Dae Kim, Yong-Gu Lee

Proceedings Volume 8458, Optical Trapping and Optical Micromanipulation IX; 84580N (2012) https://doi.org/10.1117/12.929332
Event: SPIE NanoScience + Engineering, 2012, San Diego, California, United States

Abstract

The introduction and subsequent expression of external DNA inside single living mammalian cell (transfection) can be achieved by photoporation with femtosecond laser. After photoporation, external DNA can be introduced by trapping and successive insertion of DNA coated nanoparticle in the cell using optical tweezers. To maximize the transfection efficiency, one of the major aspects is that the photoporated cell should not be damaged and cell membrane should heal itself immediately or after sometime while the cells are healed in the CO₂ incubator. Furthermore, the size of hole created as a result of photoporation should be more than the size of DNA coated nanoparticle to be inserted inside the cell. In this paper, an analysis has been done on single cell of important breast cancer cell lines named MCF-7 and MDAMB231. Size of holes created in cell membrane after photoporation has been measured and the required optimum energy with sustained cell life were determined. Using this analysis, most favorable conditions for maximum transfection efficiency can be determined.

Citation Download Citation

Waleed Muhammad, Jung-Dae Kim, and Yong-Gu Lee "Analysis of cell poration by femtosecond laser for particle insertion by optical manipulation", Proc. SPIE 8458, Optical Trapping and Optical Micromanipulation IX, 84580N (10 October 2012); https://doi.org/10.1117/12.929332

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