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24 October 2012 Optogenetic mapping of brain circuitry
George J. Augustine, Ken Berglund, Harin Gill, Carolin Hoffmann, Malvika Katarya, Jinsook Kim, John Kudolo, Li Ming Lee, Molly Lee, Daniel Lo, Ryuichi Nakajima, Min Yoon Park, Gregory Tan, Yanxia Tang, Peggy Teo, Sachiko Tsuda, Lei Wen, Su-In Yoon
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Proceedings Volume 8548, Nanosystems in Engineering and Medicine; 85483Y (2012) https://doi.org/10.1117/12.2008665
Event: SPIE Nanosystems in Engineering + Medicine, 2012, Incheon, Korea, Republic of
Abstract
Studies of the brain promise to be revolutionized by new experimental strategies that harness the combined power of optical techniques and genetics. We have mapped the circuitry of the mouse brain by using both optogenetic actuators that control neuronal activity and optogenetic sensors that detect neuronal activity. Using the light-activated cation channel, channelrhodopsin-2, to locally photostimulate neurons allows high-speed mapping of local and long-range circuitry. For example, with this approach we have mapped local circuits in the cerebral cortex, cerebellum and many other brain regions. Using the fluorescent sensor for chloride ions, Clomeleon, allows imaging of the spatial and temporal dimensions of inhibitory circuits in the brain. This approach allows imaging of both conventional "phasic" synaptic inhibition as well as unconventional "tonic" inhibition. The combined use of light to both control and monitor neural activity creates unprecedented opportunities to explore brain function, screen pharmaceutical agents, and potentially to use light to ameliorate psychiatric and neurological disorders.
© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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George J. Augustine, Ken Berglund, Harin Gill, Carolin Hoffmann, Malvika Katarya, Jinsook Kim, John Kudolo, Li Ming Lee, Molly Lee, Daniel Lo, Ryuichi Nakajima, Min Yoon Park, Gregory Tan, Yanxia Tang, Peggy Teo, Sachiko Tsuda, Lei Wen, and Su-In Yoon "Optogenetic mapping of brain circuitry", Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85483Y (24 October 2012); https://doi.org/10.1117/12.2008665
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KEYWORDS
Brain
Optogenetics
Neurons
Brain mapping
Sensors
Associative arrays
Genetics
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