LOSOM: phase relief imaging can be achieved with confocal system

Tong Peng; Hao Xie; Yichen Ding; Yiqing Lu; Dayong Jin; Peng Xi

doi:10.1117/12.2000809

11 December 2012 LOSOM: phase relief imaging can be achieved with confocal system

Tong Peng, Hao Xie, Yichen Ding, Yiqing Lu, Dayong Jin, Peng Xi

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 85531C (2012) https://doi.org/10.1117/12.2000809
Event: Photonics Asia, 2012, Beijing, China

Abstract

We reported recently that laser oblique scanning optical microscopy (LOSOM) is able to obtain a relief image in transparent sample directly. To optimize the performance of LOSOM, the parameters such as numerical aperture, the distance between the specimen and the fluorescent medium and the pinhole size are investigated in this work. A beam blocker is introduced in light path which enhances dramatically the visualization of local phase difference.

Citation Download Citation

Tong Peng, Hao Xie, Yichen Ding, Yiqing Lu, Dayong Jin, and Peng Xi "LOSOM: phase relief imaging can be achieved with confocal system", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85531C (11 December 2012); https://doi.org/10.1117/12.2000809

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