Quantitative interferometric microscopy: tool for phase imaging from 2D to 3D

Shouyu Wang; Liang Xue; Jiancheng Lai; Yang Song; Zhenhua Li

doi:10.1117/12.999342

11 December 2012 Quantitative interferometric microscopy: tool for phase imaging from 2D to 3D

Shouyu Wang, Liang Xue, Jiancheng Lai, Yang Song, Zhenhua Li

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 85531Q (2012) https://doi.org/10.1117/12.999342
Event: Photonics Asia, 2012, Beijing, China

Abstract

Phase distribution detection of cells and tissues is concerned since it is an important auxiliary method for observing biological samples. Here, in this paper, we have proposed phase retrieval algorithms dealing with microscopic interferograms in order to solve two-dimensional phase distribution. Based on phase distributions solved by phase retrieval algorithms, three-dimensional refractive index distribution of biological sample is reconstructed which could reflect inner structure of the cell. We believe these methods could be powerful tools in biological and medical fields.

Citation Download Citation

Shouyu Wang, Liang Xue, Jiancheng Lai, Yang Song, and Zhenhua Li "Quantitative interferometric microscopy: tool for phase imaging from 2D to 3D", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85531Q (11 December 2012); https://doi.org/10.1117/12.999342

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