Detection of silver ions induced morphological changes on E. coli membrane using digital holographic microscopy

Farzaneh Borji; Ali Mohebi; Ali-Reza Moradi; Sharareh Tavaddod

doi:10.1117/12.2001370

26 November 2012 Detection of silver ions induced morphological changes on E. coli membrane using digital holographic microscopy

Farzaneh Borji, Ali Mohebi, Ali-Reza Moradi, Sharareh Tavaddod

Author Affiliations +

Proceedings Volume 8556, Holography, Diffractive Optics, and Applications V; 85560N (2012) https://doi.org/10.1117/12.2001370
Event: Photonics Asia, 2012, Beijing, China

Abstract

In this work, we utilize digital holographic microscopy technique and also conventional video microscopy to detect the dynamic morphological changes of bacteria membrane in presence of silver ions. Silver ions have shown strong inhibitory effects on bacteria and can be used as antibacterial. We used E. coli as a sample and the influences of the ions were compared in terms of the variations in volume of E. coli for different concentration of silver ions in the buffer and various incubation times. In a controlled experiment using a microinjecting pump the concentration of antibacterial is increased, the movement of a single or a set of bacteria are monitored live, and successive digital holograms are recorded. The recorded holograms by digital camera can be post-processed to three dimensional reconstruction of the samples and measurement of quantitative structural changes in various interacting time of the bacteria.

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Farzaneh Borji, Ali Mohebi, Ali-Reza Moradi, and Sharareh Tavaddod "Detection of silver ions induced morphological changes on E. coli membrane using digital holographic microscopy", Proc. SPIE 8556, Holography, Diffractive Optics, and Applications V, 85560N (26 November 2012); https://doi.org/10.1117/12.2001370

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KEYWORDS

Bacteria

Digital holography

Ions

Silver

Holography

Holograms

Beam splitters

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