Effects of combined photochemical internalization and hyperthermia are sensitively dependent on radiant exposure

Steen J. Madsen; Christina Schlazer; Aaron Andersen; Stephanie Molina; Henry Hirschberg

doi:10.1117/12.2005407

8 March 2013 Effects of combined photochemical internalization and hyperthermia are sensitively dependent on radiant exposure

Steen J. Madsen, Christina Schlazer, Aaron Andersen, Stephanie Molina, Henry Hirschberg

Author Affiliations +

Proceedings Volume 8565, Photonic Therapeutics and Diagnostics IX; 85655F (2013) https://doi.org/10.1117/12.2005407
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Combination therapies of photochemical internalization (PCI) and hyperthermia (HT) were investigated in an in vitro system consisting of human glioma spheroids. Spheroids (350-400 μm dia.) were irradiated with 670 nm laser light in an incubator at temperatures ranging from 37 to 50 °C. For each temperature investigated (45 min. heating time), spheroids were divided into 5 groups: control, dark control, bleomycin-only, photodynamic therapy (PDT), and PCI. PDT and PCI spheroids were exposed to radiant exposures ranging from 0.5-3.0 J cm^-2 using an irradiance of 5 mW cm^-2. Toxicity was evaluated from spheroid growth kinetics. The combination of PCI and HT resulted in growth delays over a very narrow range of radiant exposures (1.5 – 2.5 J cm^-2) and temperatures (40 – 42 °C).

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Steen J. Madsen, Christina Schlazer, Aaron Andersen, Stephanie Molina, and Henry Hirschberg "Effects of combined photochemical internalization and hyperthermia are sensitively dependent on radiant exposure", Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85655F (8 March 2013); https://doi.org/10.1117/12.2005407

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