Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green

Yadir Guerrero; Baharak Bahmani; Bonsu Jung; Valentine Vullev; Vikas Kundra; Bahman Anvari

doi:10.1117/12.2006278

13 March 2013 Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green

Yadir Guerrero, Baharak Bahmani, Bonsu Jung, Valentine Vullev, Vikas Kundra, Bahman Anvari

Proceedings Volume 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII; 856819 (2013) https://doi.org/10.1117/12.2006278
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Our group has constructed a new type of viral nanoparticles (VNPs) from genome-depleted plant infecting brome mosaic virus (BMV) that encapsulates the FDA-approved near infrared (NIR) indocyanine green (ICG)[1]. We refer to these VNPs as optical viral ghosts (OVGs) since the constructs lack the genomic content of wild-type BMV. One of our areas of interest is the application of OVGs for real-time intraoperative NIR fluorescence imaging of small peritoneal ovarian tumor nodules. We target human epidermal growth factor receptor-2 (HER-2) expression in ovarian cancer as a biomarker associated with ovarian cancer, since its over-expression is linked to the disease’s progression to death. We functionalize the OVGs with anti-HER-2 monoclonal antibodies using reductive amination methods. We used fluorescence imaging to visualize the SKOV-3 cells (high HER-2 expression) after incubation with free ICG, OVGs, and functionalized OVGs. Our results suggest the possibility of using anti-HER2 conjugated OVGs in conjunction with cytoreductive surgery to detect small tumor nodules (<5cm) which currently are not excised during surgery.

Citation Download Citation

Yadir Guerrero, Baharak Bahmani, Bonsu Jung, Valentine Vullev, Vikas Kundra, and Bahman Anvari "Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green", Proc. SPIE 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII, 856819 (13 March 2013); https://doi.org/10.1117/12.2006278

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