High sensitive fundus autofluorescence imaging combined with speckle-free optical coherence tomography

Patrycjusz Stremplewski; Katarzyna Komar; Maciej Szkulmowski; Marta Motoczyńska; Maciej Wojtkowski

doi:10.1117/12.2006729

20 March 2013 High sensitive fundus autofluorescence imaging combined with speckle-free optical coherence tomography

Patrycjusz Stremplewski, Katarzyna Komar, Maciej Szkulmowski, Marta Motoczyńska, Maciej Wojtkowski

Proceedings Volume 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII; 857119 (2013) https://doi.org/10.1117/12.2006729
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Scattering and fluorescence images provide complementary information about the health condition of the human eye, so getting them in a single measurement, using a single device may significantly improve a quality of diagnosis as it has been already demonstrated in Spectralis (Heidelberg Eng.) OCT instrument. There is still challenge to improve quality of fundus autofluorescence (FAF) images. The biggest obstacle in obtaining in vivo images of sufficient quality is very low fluorescence signal. For eye safety reasons, and because of patient comfort, using highpower fluorescence excitation is not an adequate solution to the low signal problem. In this contribution we show a new detection method in the retinal autofluorescence imaging, which may improve the sensitivity. We used a fast modulated (up to 500 MHz) diode laser of wavelength 473 nm and detected fluorescence in the spectral range 500-680 nm by photomultiplier and lock-in amplifier. Average power of the collimated blue beam on the cornea used for FAF measurements was set to 50 μW, 10 μW, and even 4.5 μW.

Citation Download Citation

Patrycjusz Stremplewski, Katarzyna Komar, Maciej Szkulmowski, Marta Motoczyńska, and Maciej Wojtkowski "High sensitive fundus autofluorescence imaging combined with speckle-free optical coherence tomography", Proc. SPIE 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII, 857119 (20 March 2013); https://doi.org/10.1117/12.2006729

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available