Dual-modality fiber-optic imager (DFOI) for intracellular gene delivery in human cervical cancer cell

Jaepyeong Cha; Jing Zhang; Saumya Gurbani; Min Li; Jin U. Kang

doi:10.1117/12.2001636

20 March 2013 Dual-modality fiber-optic imager (DFOI) for intracellular gene delivery in human cervical cancer cell

Jaepyeong Cha, Jing Zhang, Saumya Gurbani, Min Li, Jin U. Kang

Proceedings Volume 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII; 857604 (2013) https://doi.org/10.1117/12.2001636
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The most common optical method to validate intracellular gene delivery in cancer is to detect tagged fluorescence signals from the cells. However, fluorescent detection is usually performed in vitro due to the limitation of standard microscopes. Herein, we propose a highly sensitive dual-modality fiber-optic imager (DFOI), which enables in vivo fluorescence imaging. Our system uses a coherent fiber bundle based imager capable of simultaneously performing both confocal reflectance and fluorescent microscopy. Non-viral vectors targeting human cervical cancer cells (HeLa) were used to evaluate the performance. Preliminary results demonstrated the DFOI is promising for in vivo evaluation of intracellular gene delivery.

Citation Download Citation

Jaepyeong Cha, Jing Zhang, Saumya Gurbani, Min Li, and Jin U. Kang "Dual-modality fiber-optic imager (DFOI) for intracellular gene delivery in human cervical cancer cell", Proc. SPIE 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII, 857604 (20 March 2013); https://doi.org/10.1117/12.2001636

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