An overview of time-domain diffuse fluorescence imaging: instrumentation and applications

Kenneth M. Tichauer; Robert W. Holt; Frederic Leblond; Brian W. Pogue

doi:10.1117/12.2005188

25 March 2013 An overview of time-domain diffuse fluorescence imaging: instrumentation and applications

Kenneth M. Tichauer, Robert W. Holt, Frederic Leblond, Brian W. Pogue

Proceedings Volume 8578, Optical Tomography and Spectroscopy of Tissue X; 857828 (2013) https://doi.org/10.1117/12.2005188
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Rapidly pulsed laser excitation with high-rate signal detection allows the dispersion of emitted fluorescent signals through tissue to be measured. This “time-domain” approach to measuring diffuse fluorescence data offers a wide range of advantages over conventional “continuous-wave” fluorescence imaging. While continuous-wave imaging provides a measure of only fluorescence intensity at each source-detector pairing, time-domain approaches provide fluorescence intensity throughout the whole temporal response to the pulsed-laser excitation, adding a whole new dimension to the dataset. This paper introduces the instrumentation required to collect time-domain data, dealing with complications and nuances of system and data calibration, as well as the major current applications of time-domain diffuse fluorescence imaging.

Citation Download Citation

Kenneth M. Tichauer, Robert W. Holt, Frederic Leblond, and Brian W. Pogue "An overview of time-domain diffuse fluorescence imaging: instrumentation and applications", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 857828 (25 March 2013); https://doi.org/10.1117/12.2005188

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