Identification of rolling circulating tumor cells using photoacoustic time-of-flight method

Mustafa Sarimollaoglu; Dmitry A. Nedosekin; Ekaterina I. Galanzha; Vladimir P. Zharov

doi:10.1117/12.2007964

4 March 2013 Identification of rolling circulating tumor cells using photoacoustic time-of-flight method

Mustafa Sarimollaoglu, Dmitry A. Nedosekin, Ekaterina I. Galanzha, Vladimir P. Zharov

Proceedings Volume 8581, Photons Plus Ultrasound: Imaging and Sensing 2013; 858124 (2013) https://doi.org/10.1117/12.2007964
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Existing optical techniques for in vivo measurement of blood flow velocity are not quite applicable for determination of velocity of individual cells or nanoparticles. A time-of-flight photoacoustic (PA) technique can solve this problem by measuring the transient PA signal width, which is related to the cell velocity passing the laser beam. This technique was demonstrated in vivo using an animal (mouse) model by estimating the velocity of nanoparticles, and red and white blood cells labeled with conjugated gold nanorods (GNRs) in the bloodstream. Here we describe the features and the parameters of novel modifications to the PA time-of-flight method and its new application for real-time monitoring of circulating tumor cells (CTCs), such as B16F10 melanoma. This method provided, for the first time, identification of rolling CTCs in analogy to rolling white blood cells and CTC aggregates. Specifically, monitoring of PA signal widths from CTCs in mouse ear microvessels revealed double maxima in peak-width histograms associated with the fast moving portion of CTCs in central flow and slowly rolling CTCs in analogy to white blood cells. We also developed a two-parameter plot representing PA peak amplitude and peak widths. This method allowed identification of fast-moving individual CTCs, CTC aggregates, and rolling CTCs. The discovery of rolling CTCs in relatively large blood vessels indicates a higher probability of CTC extravasations, further increasing the possibility of metastasis through rolling mechanism in addition to mechanical capturing of CTCs in small vessels.

Citation Download Citation

Mustafa Sarimollaoglu, Dmitry A. Nedosekin, Ekaterina I. Galanzha, and Vladimir P. Zharov "Identification of rolling circulating tumor cells using photoacoustic time-of-flight method", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 858124 (4 March 2013); https://doi.org/10.1117/12.2007964

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Blood

Photoacoustic spectroscopy

Tumors

In vivo imaging

Blood circulation

Ear

Melanoma

Show All Keywords

Keywords/Phrases

Search In:

Publication Years