Topical photodynamic therapy of squamous cell carcinomas in a hairless mouse model

Hong-Wei Wang; Ting Lv; Jing-Jing Li; Qingfeng Tu; Zheng Huang; Xiu-Li Wang

doi:10.1117/12.2006297

25 February 2013 Topical photodynamic therapy of squamous cell carcinomas in a hairless mouse model

Hong-Wei Wang, Ting Lv, Jing-Jing Li, Qingfeng Tu, Zheng Huang, Xiu-Li Wang

Proceedings Volume 8582, Biophotonics and Immune Responses VIII; 858204 (2013) https://doi.org/10.1117/12.2006297
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Objectives: To examine therapeutic effects of 5-aminolevulinate (ALA)-mediated photodynamic therapy (PDT) on UVB-induced cutaneous squamous cell carcinomas (SCCs) in a mouse model. Materials and methods: Cutaneous SCCs were established by UVB (280-320 nm) irradiation of hairless mice. In situ fluorescence measurement was used to monitor PpIX formation after the topical application of various concentrations of ALA cream to determine the optimal ALA dose. Therapeutic responses of SCCs to multiple sessions of ALA PDT were examined histologically and quantitatively. TUNEL staining was used to examine apoptosis caused by PDT. Results: After repeated exposure for 18 to 22 weeks (4-5 days/week), multiple nodular and verrucous hyperplasia lesions of various sizes developed at the exposed area. After four sessions of ALA PDT (8% ALA, 3 h incubation, 30 J/cm² at 20 mW/cm²) a total of 84% of complete response was achieved for small SCCs (1-4 mm, thickness <2.5 mm). TUNEL staining showed that PDT-induced apoptotic cells were distributed evenly from the basal to stratum corneum layers. Conclusions: Topical ALA PDT can trigger apoptosis in SCCs, inhibit SCC growth, and reduce the size and number of tumors in the hairless mouse model. The true clinical value of ALA PDT for the treatment of cutaneous SCC deserves further investigation.

Citation Download Citation

Hong-Wei Wang, Ting Lv, Jing-Jing Li, Qingfeng Tu, Zheng Huang, and Xiu-Li Wang "Topical photodynamic therapy of squamous cell carcinomas in a hairless mouse model", Proc. SPIE 8582, Biophotonics and Immune Responses VIII, 858204 (25 February 2013); https://doi.org/10.1117/12.2006297

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