Quantitative birefringence imaging of biological samples using quadri-wave interferometry

Sherazade Aknoun; Pierre Bon; Julien Savatier; Benoit Wattellier; Serge Monneret

doi:10.1117/12.2003622

22 February 2013 Quantitative birefringence imaging of biological samples using quadri-wave interferometry

Sherazade Aknoun, Pierre Bon, Julien Savatier, Benoit Wattellier, Serge Monneret

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871D (2013) https://doi.org/10.1117/12.2003622
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometry wave front sensor to perform quantitative linear birefringence measurements on biological samples. The system combines quantitative phase images with different excitation polarizations to create birefringence images. This technique is fast and compatible with living samples. It gives information about the local retardance and structure of their anisotropic components.

Citation Download Citation

Sherazade Aknoun, Pierre Bon, Julien Savatier, Benoit Wattellier, and Serge Monneret "Quantitative birefringence imaging of biological samples using quadri-wave interferometry", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871D (22 February 2013); https://doi.org/10.1117/12.2003622

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