The arrangement of fibrous collagen in cornea using second harmonic generation (SHG) microscopy

Yair Mega; James McLean; Ramin Zareian; Stephen Karasek; Zhenhua Lai; Charles DiMarzio

doi:10.1117/12.2001551

22 February 2013 The arrangement of fibrous collagen in cornea using second harmonic generation (SHG) microscopy

Yair Mega, James McLean, Ramin Zareian, Stephen Karasek, Zhenhua Lai, Charles DiMarzio

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881N (2013) https://doi.org/10.1117/12.2001551
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In recent years, interest in studying the components of the cornea and their arrangement, with emphasis on the corneal stroma, has expanded rapidly. By determining the corneal stroma’s organization in detail, we will understand better the relationship between its structure and functionality. Here, the cornea’s collagen lamellae were scanned using second harmonic generation (SHG) microscopy, in order to determine the orientation of fibers in different directions within a two-dimensional cross section. A unique algorithm was used to quantitatively measure the directions. Cross sections were obtained at several different depths in each sample. This work offers supplemental sectioning revelations to the methods historically used to scan at the lamella level, such as X-ray diffraction.

Citation Download Citation

Yair Mega, James McLean, Ramin Zareian, Stephen Karasek, Zhenhua Lai, and Charles DiMarzio "The arrangement of fibrous collagen in cornea using second harmonic generation (SHG) microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881N (22 February 2013); https://doi.org/10.1117/12.2001551

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