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22 February 2013 Hierarchical model of fibrillar collagen distribution for polarization-resolved SHG microscopy
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A hierarchical model of the organization of fibrillar collagen is developed and its implications on polarization-resolved second harmonic generation (SHG) microscopy are investigated. A “ground-up” approach is employed to develop the theory for understanding of the origin of SHG from fibrillar collagen. The effects of fibril ultrastructure and fibril macroscopic organization on the second-order polarization properties of fibrillar collagen are presented in conjunction with recent ab initio results performed on a collagen triple-helix model (-GLY-PRO-HYP-)n. Various tissues containing fibrillar collagen are quantified using a polarization-resolved SHG technique, termed polarization-in, polarization-out (PIPO) and interpreted in light of the aforementioned theory. The method involves varying the incident laser polarization, while monitoring the SHG intensity through an analyzer. From the SHG polarization data the orientation of the fibers, in biological tissue, can be deduced. Unique PIPO signatures are observed for different rat tissues and interpreted in terms of the collagen composition, fibril ultrastructure, and macroscopic organization. Similarities and discrepancies in the second-order polarization properties of different collagen types and ultrastructures will be presented. PIPO SHG microscopy shows promise in its ability to quantify the organization of collagen in various tissues. The ability to characterize the structure of collagen in various tissue microenvironments will aid in the study of numerous collagen related biological process, including tissue diseases, wound repair, and tumor development and progression.
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Adam E. Tuer, Margarete K. Akens, Serguei Krouglov, Daaf Sandkuijl, Brian C. Wilson, Cari M. Whyne, and Virginijus Barzda "Hierarchical model of fibrillar collagen distribution for polarization-resolved SHG microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881O (22 February 2013);

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