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22 February 2013 Multiphoton imaging to distinguish grana and starch inside an intact leaf
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We have demonstrated a straightforward and noninvasive method to identify the distribution of grana and starch within an intact leaf. Grana and starch are the major functional structures for photosynthesis and energy storage of plant, respectively. Both exhibit highly ordered molecular structures and appear as micrometer-sized granules inside chloroplasts. In order to distinguish grana and starch, we used multiphoton microscopy, with simultaneous acquisition of two photon fluorescence (2PF) and second harmonic generation (SHG) signals. Consequently, SHG is found on both grana and starch while 2PF from chlorophyll indicates the identity of grana.
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Mei-Yu Chen, Guan-Yu Zhuo, Po-Fu Chen, Pei-Chun Wu, Tzu-Ming Liu, and Shi-Wei Chu "Multiphoton imaging to distinguish grana and starch inside an intact leaf", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858822 (22 February 2013);

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