Processing and improvements in dynamic quantitative phase microscope

Katherine Creath; Goldie Goldstein

doi:10.1117/12.2008751

22 February 2013 Processing and improvements in dynamic quantitative phase microscope

Katherine Creath, Goldie Goldstein

Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 85891A (2013) https://doi.org/10.1117/12.2008751
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

This paper describes recent research and development related to data processing and imaging performance for a dynamic quantitative phase imaging microscope. This microscope provides instantaneous measurements of dynamic motions within and among live cells without labels or contrast agents. It utilizes a pixelated wire grid polarizer mask in front of the camera sensor that enables simultaneous measurement of multiple interference patterns. Optical path difference (OPD) and optical thickness (OT) data are obtained from phase images. Simulated DIC (gradient) and simulated dark field (gradient magnitude) images can be directly obtained from the phase enabling simultaneous capture of brightfield, phase contrast, quantitative phase, DIC and dark field. The OT is further processed to remove background shapes, and enhance topography. This paper presents a number of different processing routines to remove background surface shape enabling quantification of changes in cell position and volume over time. Data from a number of different moving biological organisms and cell cultures are presented.

Citation Download Citation

Katherine Creath and Goldie Goldstein "Processing and improvements in dynamic quantitative phase microscope", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 85891A (22 February 2013); https://doi.org/10.1117/12.2008751

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