Towards STED microscopy with nanometric optical sectioning

Siddharth Sivankutty; Thomas Barroca; Guillaume Dupuis; Christophe Lefumeux; Céline Mayet; Arnaud Dubois; Catherine Marquer; Sandrine Lécart; Marie-Claude Potier; Emmanuel Fort; Sandrine Lévêque-Fort

doi:10.1117/12.2004883

22 February 2013 Towards STED microscopy with nanometric optical sectioning

Siddharth Sivankutty, Thomas Barroca, Guillaume Dupuis, Christophe Lefumeux, Céline Mayet, Arnaud Dubois, Catherine Marquer, Sandrine Lécart, Marie-Claude Potier, Emmanuel Fort, Sandrine Lévêque-Fort

Author Affiliations +

Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI; 859018 (2013) https://doi.org/10.1117/12.2004883
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent marker to image well below the Rayleigh criterion. Also in combination with STED, we propose to use an alternative technique for optically sectioning fluorescent emitters close to the water-glass interface by selectively filtering the supercritical emission at the pupil plane. We discuss the instrumental development of such a system and its combination with other imaging techniques.

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Siddharth Sivankutty, Thomas Barroca, Guillaume Dupuis, Christophe Lefumeux, Céline Mayet, Arnaud Dubois, Catherine Marquer, Sandrine Lécart, Marie-Claude Potier, Emmanuel Fort, and Sandrine Lévêque-Fort "Towards STED microscopy with nanometric optical sectioning", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859018 (22 February 2013); https://doi.org/10.1117/12.2004883

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