Ultra-high frequency ultrasound biomicroscopy and high throughput cardiovascular phenotyping in a large scale mouse mutagenesis screen

Xiaoqin Liu; Richard Francis; Kimimasa Tobita; Andy Kim; Linda Leatherbury; Cecilia W. Lo

doi:10.1117/12.2004860

26 March 2013 Ultra-high frequency ultrasound biomicroscopy and high throughput cardiovascular phenotyping in a large scale mouse mutagenesis screen

Xiaoqin Liu, Richard Francis, Kimimasa Tobita, Andy Kim, Linda Leatherbury, Cecilia W. Lo

Author Affiliations +

Proceedings Volume 8593, Optical Methods in Developmental Biology; 859309 (2013) https://doi.org/10.1117/12.2004860
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Ultrasound biomicroscopy (UBM) is ideally suited for phenotyping fetal mice for congenital heart disease (CHD), as imaging can be carried out noninvasively to provide both hemodynamic and structural information essential for CHD diagnosis. Using the UBM (Vevo 2100; 40Hz) in conjunction with the clinical ultrasound system (Acuson Sequioa C512; 15Hz), we developed a two-step screening protocol to scan thousands fetuses derived from ENU mutagenized pedigrees. A wide spectrum of CHD was detected by the UBM, which were subsequently confirmed with follow-up necropsy and histopathology examination with episcopic fluorescence image capture. CHD observed included outflow anomalies, left/right heart obstructive lesions, septal/valvular defects and cardiac situs anomalies. Meanwhile, various extracardiac defects were found, such as polydactyly, craniofacial defects, exencephaly, omphalocele，cleft palate, most of which were associated with cardiac defects. Our analyses showed the UBM was better at assessing cardiac structure and blood flow profiles, while conventional ultrasound allowed higher throughput low-resolution screening. Our study showed the integration of conventional clinical ultrasound imaging with the UBM for fetal mouse cardiovascular phenotyping can maximize the detection and recovery of CHD mutants.

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Xiaoqin Liu, Richard Francis, Kimimasa Tobita, Andy Kim, Linda Leatherbury, and Cecilia W. Lo "Ultra-high frequency ultrasound biomicroscopy and high throughput cardiovascular phenotyping in a large scale mouse mutagenesis screen ", Proc. SPIE 8593, Optical Methods in Developmental Biology, 859309 (26 March 2013); https://doi.org/10.1117/12.2004860

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KEYWORDS

Ultrasonography

Fetus

Heart

Imaging systems

Blood circulation

UHF band

Arteries

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