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22 February 2013 Bioanalytics using single plasmonic nanostructures
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Plasmonic nanostructures promise to provide sensing capabilities with the potential for sensitive and robust assays in a high parallelization. We present here the use of individual nanostructures for the detection and manipulation of biomolecules such as DNA based on optical approaches [1]. The change in localized surface plasmon resonance of individual metal nanoparticles is utilized to monitor the binding of DNA directly or via DNA-DNA interaction. The influence of different size (length) as well as position (distance to the particle surface) is thereby studied [2]. Holes in a Cr layer present another interesting approach for bioanalytics. They are used to detect plasmonic nanoparticles as labels or to sense the binding of DNA on these particles. This hybrid system of hole and particle allows for simple (just using RGB-signals of a CCD [3]) but a highly sensitive (one nanoparticle sensitivity) detection. On the other hand, the binding of molecular layers around the particles can be detected using spectroscopic features of just an individual one of these systems. Besides sensing, individual plasmonic nanostructures can be also used to manipulate single biomolecular structures such as DNA. Attached particles can be used for local destruction [4] or cutting as well as coupling of energy into (and guiding along) the molecular structure [5].
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O. Stranik, T. Schneider, N. Jahr, J. Wirth, F. Garwe, A. Csaki, and W. Fritzsche "Bioanalytics using single plasmonic nanostructures", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 85950C (22 February 2013);

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