MUC1 aptamer based near infrared fluorescence probes for tumor diagnosis

Juan Zhao; Yuxiang Ma; Sisi Cui; Jie Cao; Samuel Achilefu; Yueqing Gu

doi:10.1117/12.2003364

21 February 2013 MUC1 aptamer based near infrared fluorescence probes for tumor diagnosis

Juan Zhao, Yuxiang Ma, Sisi Cui, Jie Cao, Samuel Achilefu, Yueqing Gu

Proceedings Volume 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V; 859614 (2013) https://doi.org/10.1117/12.2003364
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Mucin 1 (MUC1) is a cell surface mucin broadly expressed in mucosal tissues. The aberrant expression of MUC1 under-glycosylated forms has been reported in various carcinomas of the epithelium, such as breast, pancreatic and ovarian cancers. Using the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology, aptamers previously selected against MUC1 glycoprotein with high affinities and specificities. In this study, we developed two targeted near-infrared fluorescent probes for tumor in-vivo diagnostics using a MUC1 aptamer(APT) as targeted ligand and near-infrared fluorescent dye (ICG-Der-02) as labelling. MUC1 aptamer conjugated ICG-Der-02 (APT-ICG-Der-02) displayed a great selectivity to MUC1 positive cell line MCF7 and MCF7 xenograft-bearing nude mice. To improve the high targeting of the probe to the tumor cells, PEG, with high biocompatibility, non immunogenicity and long circulation, was conjugated to the probe .The new probe (APT-PEG-ICG-Der-02) showed better tumour uptake and clearance, and also displayed a great selectivity to MCF7 tumor-bearing nude mice. Data obtained demonstrate a high potential of the targeted near-infrared fluorescent probes in cancer early diagnosis.

Citation Download Citation

Juan Zhao, Yuxiang Ma, Sisi Cui, Jie Cao, Samuel Achilefu, and Yueqing Gu "MUC1 aptamer based near infrared fluorescence probes for tumor diagnosis", Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 859614 (21 February 2013); https://doi.org/10.1117/12.2003364

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KEYWORDS

Tumors

Luminescence

Near infrared

Rhodamine B

Magnesium

Chromatography

Confocal microscopy

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