Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers

Christian Witte; Clemens Kremer; Jonathan M. Cooper; Steven L. Neale

doi:10.1117/12.2004814

9 March 2013 Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers

Christian Witte, Clemens Kremer, Jonathan M. Cooper, Steven L. Neale

Proceedings Volume 8615, Microfluidics, BioMEMS, and Medical Microsystems XI; 86150T (2013) https://doi.org/10.1117/12.2004814
Event: SPIE MOEMS-MEMS, 2013, San Francisco, California, United States

Abstract

A versatile platform for efficient cell lysis using a combination of acoustic and electric fields in a microchannel is presented. Cell membrane disruption is triggered by electric fields inducing electroporation and then lysis. The principle of optoelectronic tweezers (OET) is applied to control the electric field strength and a surface acoustic wave transducer is attached to an OET chip to implement acoustic tweezing (AT). The system is characterized in terms of spatial control of electric fields, single cell precision and lysis times. Under continuous operation, a combination of AT and OET improves cell lysis significantly achieving for sample concentrations of 10⁶ cell/ml lysis efficiencies of > 99%.

Citation Download Citation

Christian Witte, Clemens Kremer, Jonathan M. Cooper, and Steven L. Neale "Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers", Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150T (9 March 2013); https://doi.org/10.1117/12.2004814

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