Biodistribution study of nanoparticle encapsulated photodynamic therapy drugs using multispectral imaging

Luma V. Halig; Dongsheng Wang; Andrew Y. Wang; Zhuo Georgia Chen; Baowei Fei

doi:10.1117/12.2006492

29 March 2013 Biodistribution study of nanoparticle encapsulated photodynamic therapy drugs using multispectral imaging

Luma V. Halig, Dongsheng Wang, Andrew Y. Wang, Zhuo Georgia Chen, Baowei Fei

Proceedings Volume 8672, Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging; 867218 (2013) https://doi.org/10.1117/12.2006492
Event: SPIE Medical Imaging, 2013, Lake Buena Vista (Orlando Area), Florida, United States

Abstract

Photodynamictherapy (PDT) uses a drug called a photosensitizer that is excited by irradiation with a laser light of a particular wavelength, which generates reactive singlet oxygen that damages the tumor cells. The photosensitizer and light are inert; therefore, systemic toxicities are minimized in PDT. The synthesis of novel PDT drugs and the use of nanosized carriers for photosensitizers may improve the efficiency of the therapy and the delivery of the drug. In this study, we formulated two nanoparticles with and without a targeting ligand to encapsulate phthalocyanines 4 (Pc 4) molecule and compared their biodistributions. Metastatic human head and neck cancer cells (M4e) were transplanted into nude mice. After 2-3 weeks, the mice were injected with Pc 4, Pc 4 encapsulated into surface coated iron oxide (IO-Pc 4), and IO-Pc 4 conjugated with a fibronectin-mimetic peptide (FMP-IO-Pc 4) which binds specifically to integrin β1. The mice were imaged using a multispectral camera. Using multispectral images, a library of spectral signatures was created and the signal per pixel of each tumor was calculated, in a grayscale representation of the unmixed signal of each drug. An enhanced biodistribution of nanoparticle encapsulated PDT drugs compared to non-formulated Pc 4 was observed. Furthermore, specific targeted nanoparticles encapsulated Pc 4 has a quicker delivery time and accumulation in tumor tissue than the non-targeted nanoparticles. The nanoparticle-encapsulated PDT drug can have a variety of potential applications in cancer imaging and treatment.

Citation Download Citation

Luma V. Halig, Dongsheng Wang, Andrew Y. Wang, Zhuo Georgia Chen, and Baowei Fei "Biodistribution study of nanoparticle encapsulated photodynamic therapy drugs using multispectral imaging", Proc. SPIE 8672, Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging, 867218 (29 March 2013); https://doi.org/10.1117/12.2006492

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