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11 April 2013 Microstructured optical fiber monitor for cryogenic applications
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Monitoring intracellular ice formation (IIF) as well as understanding the cellular freezing process at temperatures of nearly -40°C is beneficial to the study of cryopreservation. This paper discusses the use of optical absorption spectroscopy to examine the thermal changes occurring in water contained in cells as they reach cryogenic temperatures. The presented arrangement employs a 0.59 m all-silica steering-wheel photonic crystal fiber (SW-PCF) filled with <20uL of de-ionized water that is fusion spliced with a 1.13 m single mode fiber (SMF). The fluid filled SW-PCF is placed on a two-stage thermoelectric cooler (TEC). One end of the SW-PCF is coupled to an optical spectrum analyzer, while the SMF couples broadband light with emission peaks at 1350nm, 1450nm, 1550nm and 1650nm to the SW-PCF. Unlike our previous cryogenic freezing arrangement, a water circulating cooling system consisting of a cold plate with an attached radiator promotes operation temperatures of nearly – 40 °C. Styrofoam insulates the fiber/TEC configuration to provide thermal stability and prevent undesired ice condensation on the thermal system. A resistance temperature detector (RTD) monitored the thermal changes occurring over a range of temperatures between 5°C to -38 °C in 5 degree increments. The measured absorption spectra of the < 20μL de-ionized water sample filled PCF show absorption characteristics consistent with standard spectra for water vapor at cryogenic temperatures.
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Svetlana Filiche, Emily Battinelli, and Rosalind Wynne "Microstructured optical fiber monitor for cryogenic applications", Proc. SPIE 8693, Smart Sensor Phenomena, Technology, Networks, and Systems Integration 2013, 86930S (11 April 2013);

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