Raman spectroscopy for bacterial identification and characterization

Silvie Bernatová; Ota Samek; Zdeněk Pilát; Mojmír Šerý; Jan Ježek; Vladislav Krzyžánek; Pavel Zemánek; Filip Růžička

doi:10.1117/12.2010539

18 December 2012 Raman spectroscopy for bacterial identification and characterization

Silvie Bernatová, Ota Samek, Zdeněk Pilát, Mojmír Šerý, Jan Ježek, Vladislav Krzyžánek, Pavel Zemánek, Filip Růžička

Proceedings Volume 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 86970Y (2012) https://doi.org/10.1117/12.2010539
Event: 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2012, Ostravice, Czech Republic

Abstract

The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor.

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Silvie Bernatová, Ota Samek, Zdeněk Pilát, Mojmír Šerý, Jan Ježek, Vladislav Krzyžánek, Pavel Zemánek, and Filip Růžička "Raman spectroscopy for bacterial identification and characterization", Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970Y (18 December 2012); https://doi.org/10.1117/12.2010539

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