From microfluidic modules to an integrated Lab-on-a-chip system for the detection of Francisella tularensis

Nadine Hlawatsch; Marco Krumbholz; Anna Prüfer; Christian Moche; Holger Becker; Claudia Gärtner

doi:10.1117/12.2018049

31 May 2013 From microfluidic modules to an integrated Lab-on-a-chip system for the detection of Francisella tularensis

Nadine Hlawatsch, Marco Krumbholz, Anna Prüfer, Christian Moche, Holger Becker, Claudia Gärtner

Author Affiliations +

Proceedings Volume 8719, Smart Biomedical and Physiological Sensor Technology X; 87190C (2013) https://doi.org/10.1117/12.2018049
Event: SPIE Defense, Security, and Sensing, 2013, Baltimore, Maryland, United States

Abstract

Lab-on-a-chip (LoC) systems translating the whole process of pathogen analysis to an integrated, miniaturized, and automatically functioning microfluidic platform are generally expected to be very promising future diagnostic approaches. The development of such a LoC system for the detection of bacterial pathogens applied to the example pathogen Francisella tularensis is described in this report. To allow functional testing of the whole process cascade before final device integration, various bio-analytical steps such as cell lysis, DNA extraction and purification, continuous-flow PCR and analyte detection have been adapted to unique functional microfluidic modules. As a successive step, positively tested modules for pathogen detection have been successfully assembled to an integrated chip. Moreover, technical solutions for a smooth interaction between sample input from the outer world as well as microfluidic chip and chip driving instrument have been developed. In conclusion, a full repertoire of analytical tools have been developed and successfully tested in the concerted manner of a functionally integrated microfluidic device representing a tool for future diagnostic approaches.

Citation Download Citation

Nadine Hlawatsch, Marco Krumbholz, Anna Prüfer, Christian Moche, Holger Becker, and Claudia Gärtner "From microfluidic modules to an integrated Lab-on-a-chip system for the detection of Francisella tularensis", Proc. SPIE 8719, Smart Biomedical and Physiological Sensor Technology X, 87190C (31 May 2013); https://doi.org/10.1117/12.2018049

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