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29 May 2013 A new wireless detection device for the in-situ identification of Salmonella Typhimurium
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Abstract
This paper presents a new device and method for the in-situ detection of Salmonella Typhimurium on tomato surfaces. This real-time in-situ detection was accomplished with phage-based magnetoelastic (ME) biosensors on fresh food surfaces. The E2 phage from a landscape phage library serves as the bio-recognition element that has the capability of binding specifically with S. Typhimurium. This mass-sensitive ME biosensor is wirelessly actuated into mechanical resonance by an externally applied time-varying magnetic field. When the biosensor binds with S. Typhimurium, the mass of the sensor increases, resulting in a decrease in the sensor's resonant frequency. Until now, ME sensors had to be collected from the tomato surface where they are exposed to S. Typhimurium and inserted into a measurement coil for the detection of the bacterium. In contrast, the newly designed test device allows the whole detection process to take place directly on the tomato. Changes in resonant frequency over time due to the accumulation of S. Typhimurium on the sensor were measured and are presented. Real-time in-situ detection of 20 minutes was achieved. In addition, this new methodology effectively decreases the measurement error and enables the simultaneous detection of multiple pathogens.
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Yating Chai, Howard C. Wikle, Mi-kyung Park, Shin Horikawa, Xie Hong, and Bryan A. Chin "A new wireless detection device for the in-situ identification of Salmonella Typhimurium", Proc. SPIE 8721, Sensing for Agriculture and Food Quality and Safety V, 87210B (29 May 2013); https://doi.org/10.1117/12.2015080
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