Multiplexed detection of aquaculture fungicides using a pump-free optofluidic SERS microsystem

Soroush H. Yazdi; Ian M. White

doi:10.1117/12.2015528

29 May 2013 Multiplexed detection of aquaculture fungicides using a pump-free optofluidic SERS microsystem

Soroush H. Yazdi, Ian M. White

Proceedings Volume 8722, Fiber Optic Sensors and Applications X; 87220V (2013) https://doi.org/10.1117/12.2015528
Event: SPIE Defense, Security, and Sensing, 2013, Baltimore, Maryland, United States

Abstract

In this paper, we present an optofluidic SERS microsystem suitable for on-site detection of multiplexed analytics in the field. We show simultaneous detection of three fungicides that are highly regulated in aquaculture. The optofluidic SERS microsystem shows improved portability since it does not require a bulky pump for sample loading. The sample is simply drawn into the microchannel using a pipette. Additionally, two fiber optic cables are inserted into the device for sample excitation and photon collection. The fiber optic cables, aligned into the detection zone, eliminate the need for microscope alignment required in traditional SERS detection. The detection zone of the device consists of a porous matrix of packed silica microspheres that traps silver nanoparticles and adsorbed analyte molecules. The sample is passively concentrated into the matrix as it is loaded by applying negative pressure from the outlet with a pipette. The concentrating matrix has been shown to amplify the SERS signal by up to four orders of magnitude as compared to SERS in an open microfluidic channel. We were able to detect as low as 5 ppm methyl parathion, 0.1 ppb malachite green, and 5 ppb thiram simultaneously.

Citation Download Citation

Soroush H. Yazdi and Ian M. White "Multiplexed detection of aquaculture fungicides using a pump-free optofluidic SERS microsystem", Proc. SPIE 8722, Fiber Optic Sensors and Applications X, 87220V (29 May 2013); https://doi.org/10.1117/12.2015528

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