Sterile measurement on the characteristics of chlorophyll fluorescence in plantlets in vitro preserved under low temperature condition

Yanyou Wu; Deke Xing

doi:10.1117/12.2020252

19 March 2013 Sterile measurement on the characteristics of chlorophyll fluorescence in plantlets in vitro preserved under low temperature condition

Yanyou Wu, Deke Xing

Author Affiliations +

Proceedings Volume 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering; 87621O (2013) https://doi.org/10.1117/12.2020252
Event: Third International Conference on Photonics and Image in Agriculture Engineering (PIAGENG 2013), 2013, Sanya, China

Abstract

Micro-environment such as temperature influenced the growth and quality of plantlets in vitro. Chlorophyll fluorescence (ChlF) parameter is an important one for photosynthesis capacity in plant. The modulated chlorophyll fluorescence imaging system can be used for obtaining ChlF imaging and signal of plantlet in vitro because the container has light permeability. Therefore, the biological activity and growth condition of plantlet in vitro can be estimated by sterilely measuring the characteristics of chlorophyll fluorescence. This study determined the parameter of chlorophyll fluorescence in Orychophragmus violaceus plantlets in vitro preserved under different temperature levels (0, 4, and 8°C). The results showed that photosynthesis capacity in Orychophragmus violaceus plantlets in vitro preserved under 4°C condition were higher than that under 0°C or 8°C conditions. The plantlets in vitro preserved under 4°C condition maintained a high vitality to be subcultured.

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Yanyou Wu and Deke Xing "Sterile measurement on the characteristics of chlorophyll fluorescence in plantlets in vitro preserved under low temperature condition", Proc. SPIE 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering, 87621O (19 March 2013); https://doi.org/10.1117/12.2020252

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KEYWORDS

In vitro testing

Luminescence

Photosynthesis

Imaging systems

Temperature metrology

Fermium

Modulation

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