Optical spectroscopy for differentiation of liver tissue under distinct stages of fibrosis: an ex vivo study

D. A. Fabila; L. F. Hernández; J. de la Rosa; S. Stolik; U. D. Arroyo-Camarena; M. D. López-Vancell; G. Escobedo

doi:10.1117/12.2021716

18 November 2013 Optical spectroscopy for differentiation of liver tissue under distinct stages of fibrosis: an ex vivo study

D. A. Fabila, L. F. Hernández, J. de la Rosa, S. Stolik, U. D. Arroyo-Camarena, M. D. López-Vancell, G. Escobedo

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Proceedings Volume 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications; 8785GD (2013) https://doi.org/10.1117/12.2021716
Event: 8th Ibero American Optics Meeting/11th Latin American Meeting on Optics, Lasers, and Applications, 2013, Porto, Portugal

Abstract

Liver fibrosis is the decisive step towards the development of cirrhosis; its early detection affects crucially the diagnosis of liver disease, its prognosis and therapeutic decision making. Nowadays, several techniques are employed to this task. However, they have the limitation in estimating different stages of the pathology. In this paper we present a preliminary study to evaluate if optical spectroscopy can be employed as an auxiliary tool of diagnosis of biopsies of human liver tissue to differentiate the fibrosis stages. Ex vivo fluorescence and diffuse reflectance spectra were acquired from biopsies using a portable fiber-optic system. Empirical discrimination algorithms based on fluorescence intensity ratio at 500 nm and 680 nm as well as diffuse reflectance intensity at 650 nm were developed. Sensitivity and specificity of around 80% and 85% were respectively achieved. The obtained results show that combined use of fluorescence and diffuse reflectance spectroscopy could represent a novel and useful tool in the early evaluation of liver fibrosis.

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D. A. Fabila, L. F. Hernández, J. de la Rosa, S. Stolik, U. D. Arroyo-Camarena, M. D. López-Vancell, and G. Escobedo "Optical spectroscopy for differentiation of liver tissue under distinct stages of fibrosis: an ex vivo study", Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 8785GD (18 November 2013); https://doi.org/10.1117/12.2021716

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