Potential of chlorophyll fluorescence imaging for assessing bio-viability changes of biodeteriogen growths on stone monuments

I. Osticioli; M. Mascalchi; D. Pinna; S. Siano

doi:10.1117/12.2020563

30 May 2013 Potential of chlorophyll fluorescence imaging for assessing bio-viability changes of biodeteriogen growths on stone monuments

I. Osticioli, M. Mascalchi, D. Pinna, S. Siano

Author Affiliations +

Proceedings Volume 8790, Optics for Arts, Architecture, and Archaeology IV; 879003 (2013) https://doi.org/10.1117/12.2020563
Event: SPIE Optical Metrology 2013, 2013, Munich, Germany

Abstract

A systematic study on the use of Chlorophyll Fluorescence (CF) imaging in Pulsed Amplitude Modulated (PAM) for assessing viability changes of biodeteriogen on stone artifacts has been carried out. The experimentation has been performed on different phototrophic organisms of gravestone slabs from the monumental British Cemetery of Florence (Italy). Since the viability of these organisms and then their chlorophyll fluorescence emission is strongly dependent on the environmental conditions, a preliminary study on the effects of local patterns during the season was carried out. The trend of the fluorescence quantum yield (QY_max) at different dark adapted times in different periods of the year was determined. The results achieved in our work proves the effectiveness of the CF-PAM imaging for in situ lichen characterizations in conservation studies and defines an optimized application protocol.

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I. Osticioli, M. Mascalchi, D. Pinna, and S. Siano "Potential of chlorophyll fluorescence imaging for assessing bio-viability changes of biodeteriogen growths on stone monuments", Proc. SPIE 8790, Optics for Arts, Architecture, and Archaeology IV, 879003 (30 May 2013); https://doi.org/10.1117/12.2020563

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