Speckle based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells

Mark Golberg; Dror Fixler; Asher Shainberg; Sharon Zlochiver; Vicente Micó; Javier Garcia; Yevgeny Beiderman; Zeev Zalevsky

doi:10.1117/12.2021642

23 May 2013 Speckle based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells

Mark Golberg, Dror Fixler, Asher Shainberg, Sharon Zlochiver, Vicente Micó, Javier Garcia, Yevgeny Beiderman, Zeev Zalevsky

Author Affiliations +

Proceedings Volume 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials; 87921E (2013) https://doi.org/10.1117/12.2021642
Event: SPIE Optical Metrology 2013, 2013, Munich, Germany

Abstract

In this manuscript we propose optical lensless configuration for a remote non-contact measuring of mechanical contractions of vast number of cardiac myocytes. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the generated secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (approximately one thousand cells) and to extract the statistical data of their movement at once. The presented experimental results also include investigation the effect of isoproteranol on cells contraction process.

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Mark Golberg, Dror Fixler, Asher Shainberg, Sharon Zlochiver, Vicente Micó, Javier Garcia, Yevgeny Beiderman, and Zeev Zalevsky "Speckle based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells", Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 87921E (23 May 2013); https://doi.org/10.1117/12.2021642

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KEYWORDS

Heart

Speckle

Speckle pattern

Microscopes

In vitro testing

Inspection

Biomedical optics

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