Application of a laser trap as a viscometer

James Cooper; Rance Solomon; Samuel Elrod; Taylor Barnes; Cameron Crawford; Anthony Farone; Mary Farone; Daniel Erenso

doi:10.1117/12.2033507

17 June 2013 Application of a laser trap as a viscometer

James Cooper, Rance Solomon, Samuel Elrod, Taylor Barnes, Cameron Crawford, Anthony Farone, Mary Farone, Daniel Erenso

Proceedings Volume 8797, Advanced Microscopy Techniques III; 87970H (2013) https://doi.org/10.1117/12.2033507
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

A laser tweezer (LT) along with advanced imaging techniques has been widely applied to manipulate and study living as well as nonliving microscopic objects. In this study we present yet another novel application of LTs for a precise measurement of the viscosities of fluids in a micro-volume flow. We have demonstrated this novel application by measuring the viscosity of a fetal bovine serum (FBS) using a LT constructed from a single intensity gradient laser trap. By calibrating the LT using dielectric silica micro-beads in a fluid with a known viscosity, specifically water, and by suspending same size of silica beads in the FBS and trapping with the same trap, we have determined the viscosity of the FBS at different temperatures. We have used the relationship between the trapping and Stoke’s drag force for a constant drag speed to determine the viscosity. We have also analyzed the viscosities determined in comparison with corresponding viscosities measured using an Ostwald viscometer.

Citation Download Citation

James Cooper, Rance Solomon, Samuel Elrod, Taylor Barnes, Cameron Crawford, Anthony Farone, Mary Farone, and Daniel Erenso "Application of a laser trap as a viscometer", Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970H (17 June 2013); https://doi.org/10.1117/12.2033507

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