Oligonucleotide switches and nanomaterials for intracellular mRNA sensing

S. Tombelli; M. Ballestri; G. Giambastiani; A. Giannetti; A. Guerrini; G. Sotgiu; C. Trono; G. Tuci; G. Varchi; F. Baldini

doi:10.1117/12.2033185

18 June 2013 Oligonucleotide switches and nanomaterials for intracellular mRNA sensing

S. Tombelli, M. Ballestri, G. Giambastiani, A. Giannetti, A. Guerrini, G. Sotgiu, C. Trono, G. Tuci, G. Varchi, F. Baldini

Author Affiliations +

Proceedings Volume 8798, Clinical and Biomedical Spectroscopy and Imaging III; 879802 (2013) https://doi.org/10.1117/12.2033185
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.

Citation Download Citation

S. Tombelli, M. Ballestri, G. Giambastiani, A. Giannetti, A. Guerrini, G. Sotgiu, C. Trono, G. Tuci, G. Varchi, and F. Baldini "Oligonucleotide switches and nanomaterials for intracellular mRNA sensing", Proc. SPIE 8798, Clinical and Biomedical Spectroscopy and Imaging III, 879802 (18 June 2013); https://doi.org/10.1117/12.2033185

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