Gold nanoparticle mediated laser transfection for high-throughput antisense applications

S. Kalies; D. Heinemann; M. Schomaker; T. Birr; T. Ripken; H. Meyer

doi:10.1117/12.2032385

24 June 2013 Gold nanoparticle mediated laser transfection for high-throughput antisense applications

S. Kalies, D. Heinemann, M. Schomaker, T. Birr, T. Ripken, H. Meyer

Proceedings Volume 8803, Medical Laser Applications and Laser-Tissue Interactions VI; 880309 (2013) https://doi.org/10.1117/12.2032385
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

The delivery of antisense structures, like siRNA, is beneficial for new therapeutic approaches in regenerative sciences. Optical transfection techniques enable high spatial control combined with minimal invasive treatment of cells due to the use of short laser pulses. However, single cell laser transfection by a tightly focused laser beam, for example femtosecond laser transfection, has the major drawback of low throughput. Compared to this, high-throughput in laser transfection is possible by applying gold nanoparticles irradiated by a weakly focused laser beam scanning over the cell sample. Herein, we show the delivery of antisense molecules and demonstrate the minimal cytotoxicity of a method called gold nanoparticle mediated (GNOME) laser transfection. A 532 nm microchip laser in conjugation with 200 nm gold nanoparticles at a concentration of 0.5 μg/cm² is used. In addition to antisense molecules, the uptake of dextrans of several sizes is analyzed.

Citation Download Citation

S. Kalies, D. Heinemann, M. Schomaker, T. Birr, T. Ripken, and H. Meyer "Gold nanoparticle mediated laser transfection for high-throughput antisense applications", Proc. SPIE 8803, Medical Laser Applications and Laser-Tissue Interactions VI, 880309 (24 June 2013); https://doi.org/10.1117/12.2032385

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