Spectral investigations on binding of DNA-CTMA complex with tetrameric copper phthalocyanines

Narayanan Venkat; Joy E. Haley; Rachel Swiger; Lei Zhu; Xiaoliang Wei; Fahima Ouchen; James G. Grote

doi:10.1117/12.2027207

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8 October 2013 Spectral investigations on binding of DNA-CTMA complex with tetrameric copper phthalocyanines

Narayanan Venkat, Joy E. Haley, Rachel Swiger, Lei Zhu, Xiaoliang Wei, Fahima Ouchen, James G. Grote

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Proceedings Volume 8817, Nanobiosystems: Processing, Characterization, and Applications VI; 88170E (2013) https://doi.org/10.1117/12.2027207
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

The binding of DNA-CTMA (Deoxyribonucleic acid-cetyltrimethylammonium) complex with two tetrameric Copper Phthalocyanine (CuPc) systems, substituted with carboxylic acid (CuPc-COOH) and derivatized further as an imidazolium salt (CuPc-COOR), was investigated in dimethylsulfoxide (DMSO) solutions using UV/Visible Spectroscopy. Absorbance changes at 685 nm (Q band of the CuPc) were monitored as a function of DNA-CTMA added to the dye solution and stock concentrations of DNA-CTMA in DMSO were varied to facilitate observation of the full binding process. Our findings indicated that while binding with DNA-CTMA was more well-defined in the case of CuPc-COOH, the binding profile of the CuPc-COOR showed initial growth followed by decay in its Q-band absorbance which was indicative of a more complex binding mechanism involving the dye and DNA-CTMA. Preliminary findings from photophysical studies involving the CuPc tetramers and DNA-CTMA are also discussed in this paper.

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Narayanan Venkat, Joy E. Haley, Rachel Swiger, Lei Zhu, Xiaoliang Wei, Fahima Ouchen, and James G. Grote "Spectral investigations on binding of DNA-CTMA complex with tetrameric copper phthalocyanines", Proc. SPIE 8817, Nanobiosystems: Processing, Characterization, and Applications VI, 88170E (8 October 2013); https://doi.org/10.1117/12.2027207

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