1D-scanning addressable multiregional multifocal multiphoton microscopy

Wan Qin; Yonghong Shao; Honghai Liu; Xiang Peng; Hanben Niu; Bruce Z. Gao

doi:10.1117/12.2024325

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28 September 2013 1D-scanning addressable multiregional multifocal multiphoton microscopy

Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, Bruce Z. Gao

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Proceedings Volume 8845, Ultrafast Imaging and Spectroscopy; 884517 (2013) https://doi.org/10.1117/12.2024325
Event: SPIE Optical Engineering + Applications, 2013, San Diego, California, United States

Abstract

We developed a novel addressable multiregional multiphoton microscope that employs a fast one-dimensional discrete-line scanning approach based on a spatial light modulator (SLM). The phase-only SLM shapes an incoming mode-locked, near-infrared Ti:sapphire laser beam into multiple specific discrete-lines, which are designed according to the sizes and locations of the target samples. Only the target-sample areas of are scanned one-dimensionally, resulting in an efficient use of the laser’s power. Compared with conventional multiphoton microscopies, this technique shortens scanning time and minimizes photodamage by concentrating scanning energy and dwell time on the areas of interest. Additionally, our discrete-line-focus design eliminates the cross-talk that occurs in conventional one-dimensional line-scanning multiphoton microscopes, thus enhancing the lateral and axial resolutions of the line-scanning imaging system.

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Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, and Bruce Z. Gao "1D-scanning addressable multiregional multifocal multiphoton microscopy", Proc. SPIE 8845, Ultrafast Imaging and Spectroscopy, 884517 (28 September 2013); https://doi.org/10.1117/12.2024325

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