Development of an in-vacuum x-ray microscope with cryogenic sample cooling for beamline P11 at PETRA III

Alke Meents; Bernd Reime; Nicolas Stuebe; Pontus Fischer; Martin Warmer; Dennis Goeries; Jan Roever; Jan Meyer; Janine Fischer; Anja Burkhardt; Ismo Vartiainen; Petri Karvinen; Christian David

doi:10.1117/12.2027303

26 September 2013 Development of an in-vacuum x-ray microscope with cryogenic sample cooling for beamline P11 at PETRA III

Alke Meents, Bernd Reime, Nicolas Stuebe, Pontus Fischer, Martin Warmer, Dennis Goeries, Jan Roever, Jan Meyer, Janine Fischer, Anja Burkhardt, Ismo Vartiainen, Petri Karvinen, Christian David

Author Affiliations +

Proceedings Volume 8851, X-Ray Nanoimaging: Instruments and Methods; 88510K (2013) https://doi.org/10.1117/12.2027303
Event: SPIE Optical Engineering + Applications, 2013, San Diego, California, United States

Abstract

Beamline P11 at PETRA III is dedicated to structural investigations of biological samples. It provides two experimental stations, one for macromolecular crystallography and one for X-ray microscopy. The microscope will provide full field Zernike phase contrast and scanning microscopy both in 2D and in tomographic mode. Full field microscopy with a field of view of 50 x 50 μm² will allow to generate an overview of the sample and to select regions of interest for later inspection of the element distribution by X-ray fluorescence and diffraction in scanning mode. Central part of the microscope is an inhouse developed flexure based x,y,z scanner on top of a rotation stage. The scanner is operated in closed loop with piezo motors, has a travel range of 4 mm in horizontal and of 3 mm in vertical direction. With laser interferometers for closed loop operation a positioning accuracy of better than 5 nm is achieved in all directions. For precise sample rotation an in-vacuum air-bearing has been developed. An open bore in the center of the air-bearing allows cryogenic sample cooling by a cold He or N₂ gas stream. Different optical elements such as beam defining pinholes, a condensor, zone plates, OSA, phase rings, etc. can be centered in the beam path by piezomotor driven x,y flexure elements mounted on a rail system which allows further positioning along the beam path. Different 2D detectors and two fluoresence detectors can be attached to the microscope.

Citation Download Citation

Alke Meents, Bernd Reime, Nicolas Stuebe, Pontus Fischer, Martin Warmer, Dennis Goeries, Jan Roever, Jan Meyer, Janine Fischer, Anja Burkhardt, Ismo Vartiainen, Petri Karvinen, and Christian David "Development of an in-vacuum x-ray microscope with cryogenic sample cooling for beamline P11 at PETRA III", Proc. SPIE 8851, X-Ray Nanoimaging: Instruments and Methods, 88510K (26 September 2013); https://doi.org/10.1117/12.2027303

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