Real-time detection of neurite outgrowth using microfluidic device

Samhwan Kim; Jongmoon Jang; Hongsoo Choi; Cheil Moon

doi:10.1117/12.2018667

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20 May 2013 Real-time detection of neurite outgrowth using microfluidic device

Samhwan Kim, Jongmoon Jang, Hongsoo Choi, Cheil Moon

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Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 887906 (2013) https://doi.org/10.1117/12.2018667
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.

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Samhwan Kim, Jongmoon Jang, Hongsoo Choi, and Cheil Moon "Real-time detection of neurite outgrowth using microfluidic device", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887906 (20 May 2013); https://doi.org/10.1117/12.2018667

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