LUSH-based SPR sensor for the detection of alcohols and pheromone

Hui-Chong Lau; Yeon-Kyung Lee; Jae-Young Kwon; Young-Soo Sohn; Jeong Ok Lim

doi:10.1117/12.2018709

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20 May 2013 LUSH-based SPR sensor for the detection of alcohols and pheromone

Hui-Chong Lau, Yeon-Kyung Lee, Jae-Young Kwon, Young-Soo Sohn, Jeong Ok Lim

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Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 88790F (2013) https://doi.org/10.1117/12.2018709
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

Protein is a widely used sensing substrate in the biosensing technology. In the study conducted here, we used odorant binding protein, LUSH from Drosophila as a biosensing substrate in a miniaturized surface plasmon resonance (SPR) sensor. LUSH contains the specific alcohols binding sites, which mediates the detection of alcohols and pheromone. We first modified the surface of the gold sensor chip using the self assembled monolayer in the chloroform solution. The saturated concentration was determined prior to the detection of alcohols and pheromone at various concentrations. The results showed that the LUSH was saturated at 1000 μg/ml on the gold sensor chip. The detection response of LUSH was significant at higher concentration of alcohols. LUSH detected ethanol at concentration ≥50%; propanol was detected at ≥25% whereas pheromone was detected at ≥1.25 μg/μl. The results provide some fundamental information on the potential use of LUSH-based SPR as a simple and easy protein-based sensor in the near future.

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Hui-Chong Lau, Yeon-Kyung Lee, Jae-Young Kwon, Young-Soo Sohn, and Jeong Ok Lim "LUSH-based SPR sensor for the detection of alcohols and pheromone", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790F (20 May 2013); https://doi.org/10.1117/12.2018709

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